Mol Biol & Biochem Protocols|書籍線上App不用買

Molecular Biology and Biochemistry Protocols Package v1.0

* Over 70 most common protocols and buffer recipes used in the Molecular Biology, Biochemistry and Cell Biology labs.

* NEW: The protocols can be edited in Word and saved.

* The program can be copied to SD card.

* No internet connection is required to use the program (100% offline).

* The package will continue to grow and regular updates will be provided for free.

* If you would like to add any protocol to this package, please email your suggestion/protocol to info@proteinguru.com

* Note: The package can be uninstalled and refunded within the first 15 minutes of purchase (Android Market rules).

Table of Contents for v1.0 (Jan 2012 release):

DNA World:

Agarose gel electrophoresis and PCR Check

Colony PCR from yeast colonies (application: Y2H screens)

Touch-down PCR protocol

10 Rules of Primer Design

Restriction Digestion Setup

Qiagen’s PCR purification column

Ligation Reaction Setup

T-vector for cloning PCR products

Phenol-Chloroform DNA isolation

Yeast Transformation

CIP-treatment

Plasmid Prep (classical protocol)

Miniprep (Qiagen kit)

Silver Staining of DNA Gels

LB-Ampicillin Plates

Chemically Competent E. coli Preparation

Transformation of the Chemically Competent E. coli Cells

Transformation - 5min Shortcut for Ampicillin Resistant Plasmids

Chromatin Immunoprecipitation (ChIP) Protocol

RNA World:

siRNA resuspension

RNA isolation from cultured cells by using RNeasy kit (Qiagen)

RT-PCR with oligo(dT) primers

Protein World:

General IP protocol

Protein expression in E. coli.

Purification of His-tagged proteins from E. coli

SDS-PAGE

Coomassie Brilliant Blue Staining of Protein Gels

Silver Staining of Protein Gels

Western Blotting

Western Blot Stripping

Preparation of Enhanced ChemiLuminescence (ECL) Substrate Buffers

IP-kinase protocol for P81 paper assay

IP-kinase protocol for autoradiography

Preparation of SILAC media

Sample Preparation for phospho-SILAC (global phosphoproteomic analysis)

Phospho-SILAC Analysis using MAXQUANT

Cell World:

Freezing cells

Hoechst staining

Immunofluorescence

Propidium Iodide Staining of Cells for Cell Cycle Analysis by FACS

Transfection of cells using PEI

C. elegans World:

Egg prep protocol

Myosin purification from C. Elegans

Buffer and Solution Recipes:

PBS

TBS

TBST

5X SDS-PAGE Sample Buffer

6X Agarose Loading Buffer

40% acryl stock (29:1) (for DNA gels)

20% paraformaldehyde stock

0.5M ATP stock

1M DTT

0.5M EDTA

50X TAE

10X TBE

TE

20X SSC

LB medium

SOC medium

M9 Buffer

Lysis / IP Buffer

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